Prediction of enzyme action for extraction of antimicrobial substances from Sus scrofa and Bos taurus
https://doi.org/10.21323/2618-9771-2024-7-1-125-136
Abstract
The study of antimicrobial compounds of animal origin, particularly antimicrobial peptides (AMPs), is a current research topic. However, extracting endogenous AMPs is a challenging process and requires the application of targeted enzymatic processing principles based on knowledge of the structure of prepropeptide molecules — precursors of AMPs. In this study, a search was conducted for antimicrobial peptides present in Sus scrofa and Bos taurus organisms, as well as their precursors, using The Antimicrobial Peptide Database and UniProtKB databases. In the amino acid sequences of prepropeptides, the sequences of the mature peptides were found, and cleavage sites for trypsin, bacterial collagenase (type I), and neutrophil elastase were determined. As a result of the search for antimicrobial compounds in The Antimicrobial Peptide Database, 18 antimicrobial peptides from Sus scrofa and 40 antimicrobial peptides from Bos taurus were identified. Based on the results of determining cleavage sites in AMP precursors, enzymes were ranked from less preferred to more preferred for AMP release as follows: bacterial collagenase (type I) ≤ trypsin < neutrophil elastase. This order is justified not only by the number of suitable cleavage sites and their accuracy but also by the action of enzymes within mature AMPs: it is important to consider that enzymes can “cut” the peptides themselves, thereby reducing their antimicrobial activity. The bioinformatics analysis conducted is applicable for both primary screening of raw material potential and determining of suitable enzymes for extracting antimicrobial compounds from Sus scrofa and Bos taurus organisms.
Keywords
antimicrobial peptides,
trypsin,
elastase,
collagenase,
cleavage site,
prepropeptide,
bioinformatic analysis
Supporting agencies: The article was published as part of the research topic No. FGUS-2024–0003 of the state assignment of the V. M. Gorbatov Federal Research Center for Food Systems of RAS.
About the Authors
E. K. Polishchuk
V. M. Gorbatov Federal Research Center for Food Systems
Russian Federation
Russian Federation
Ekaterina K. Polishchuk, Junior Researcher, Experimental Clinic — Research Laboratory of Biologically Active Substances of an Animal Origin
26, Talalikhina str., 109316, Moscow, Russia
Tel.: +7–495–676–95–11 (129)
E. A. Kotenkova
V. M. Gorbatov Federal Research Center for Food Systems
Russian Federation
Russian Federation
Elena A. Kotenkova, Candidate of Technical Sciences, Senior Researcher, Experimental Clinic — Research Laboratory of Biologically Active Substancesof an Animal Origin
26, Talalikhina str., 109316, Moscow, Russia
Tel.: +7–495–676–95–11 (129)
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Review
For citations:
Polishchuk E.K., Kotenkova E.A. Prediction of enzyme action for extraction of antimicrobial substances from Sus scrofa and Bos taurus. Food systems. 2024;7(1):125-136. (In Russ.) https://doi.org/10.21323/2618-9771-2024-7-1-125-136
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